pgk1 specific inhibitor ng52  (MedChemExpress)

Journal: International Journal of Biological Sciences

Article Title: PGK1 Regulates Oxidative Stress in Gestational Diabetes Mellitus through the Estradiol-Keap1-Nrf2 Pathway

doi: 10.7150/ijbs.113728

Figure Lengend Snippet: PGK1 inhibition improves pregnancy outcomes in vivo and alleviates trophoblast OS in vitro . Schematic diagram of mouse treatments. C) Representative images (B) and fetal quantification (C) of the control group, GDM group, and NG52 group; n=5. (D) H&E staining of mouse placental sections. Up: Overview (scale bar = 1,000 μm). Down: High magnification (scale bar = 100 μm); n = 5. (E) Representative Western blot images of Keap1, Nrf2, Ho-1, and NQO1 in the three groups. (F) IF analysis of ROS levels (red) in mouse placental tissues, and nuclei were counterstained with DAPI (blue); scale bar = 100 μm. Representative IHC staining of 4-HNE in NC, GDM, and NG52-treated mouse placentas. Up: Overview (scale bar = 1 mm). Down: High magnification (scale bar = 100 μm). Representative Western blot images of Keap1, p-Nrf2, Nrf2, Ho-1, and NQO1 levels in HTR8/SVneo cells. OCR measurements. The cells were treated with oligomycin (1.5 μM), FCCP (1 μM) or Rot/AA (0.5 μM) as indicated. The morphology of mitochondria were detected by staining with MitoSox™ red; scale bar = 20 μm. Representative fluorescence images showing MCMP in the various groups; scale bar = 20 μm. Representative fluorescence images showing DCFH-DA (green) staining in HTR8/SVneo cells, and nuclei were counterstained with Hoechst (blue); scale bar = 250 μm. Representative fluorescence images showing MitoSOX staining (red) of HTR8/SVneo cells and nuclei counterstained with Hoechst (blue); scale bar = 100 μm. * represents p < 0.05, ** represents p < 0.01 and ns represents p > 0.05.

Article Snippet: The GDM group (n = 15) was administered vehicle, the NG52 group (n = 5) was administered NG52 (100 mg/kg; MCE, USA) dissolved in 0.5% CMC-Na (Solarbio, China) by gavage, and the estradiol group (n = 5) was intraperitoneally injected with estradiol solution (0.2 mg/kg/day; MCE, USA) dissolved in olive oil from GD 9.5 to GD 18.5.

Techniques: Inhibition, In Vivo, In Vitro, Control, Staining, Western Blot, Immunohistochemistry, Fluorescence

Journal: International Journal of Biological Sciences

Article Title: PGK1 Regulates Oxidative Stress in Gestational Diabetes Mellitus through the Estradiol-Keap1-Nrf2 Pathway

doi: 10.7150/ijbs.113728

Figure Lengend Snippet: HG-induced adverse pregnancy outcomes can be partly ameliorated by supplementation with estradiol. (A) Schematic diagram of the grouping and treatment of the mice. (B) ELISA was performed to measure estradiol levels in mouse serum across five groups: the VM, NC, GDM, NG52, and estradiol-treated groups; n = 5. (C-D) Representative images (C) and quantification (D) of fetuses in the NC, GDM, and estradiol groups; n = 5. (E) H&E staining of mouse placentas. Up: Overview (scale bar = 1,000 μm). Down: High magnification (scale bar = 100 μm). (F) Representative Western blot images of Nrf2, Ho-1, and NQO1 in the three groups are shown. (G) Representative Western blot images of Keap1 monomers and dimers in the three groups. (H) Representative IF images of PGK1 (red), Keap1 (yellow) and Nrf2 (green) in mouse placental tissues. Nuclei were stained with DAPI (blue); scale bar = 200 μm. (I) Representative IF images of ROS (red) in mouse placental tissues. Nuclei were stained with DAPI (blue); scale bar = 100 μm. (J) Representative IHC staining of 4-HNE in NC, GDM, and estradiol-treated mouse placentas. Up: Overview (scale bar = 1 mm). Down: High magnification (scale bar = 100 μm). * represents p < 0.05, ** represents p < 0.01, *** represents p < 0.001, **** represents p < 0.0001 and ns represents p > 0.05.

Article Snippet: The GDM group (n = 15) was administered vehicle, the NG52 group (n = 5) was administered NG52 (100 mg/kg; MCE, USA) dissolved in 0.5% CMC-Na (Solarbio, China) by gavage, and the estradiol group (n = 5) was intraperitoneally injected with estradiol solution (0.2 mg/kg/day; MCE, USA) dissolved in olive oil from GD 9.5 to GD 18.5.

Techniques: Enzyme-linked Immunosorbent Assay, Staining, Western Blot, Immunohistochemistry